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DNA and RNA quality control

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Sample quality is critical in genomic experiments. Performing sample QC before, during and after library preparation delivers valuable information that is vital to the overall success of the sequencing results.

At GCF, we place high value on quality control at every stage of our process to ensure delivery of high-quality data.

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• Input sample QC: By determining the input sample (DNA/RNA) quality before library preparation provide the necessary information to adapt library preparation protocols according to the sample鈥檚 concentration, quality and integrity
• Intermediate sample QC: Evaluating the quality of intermediate products during library preparation ensures that only samples meeting the required criteria are selected
• Final library QC: A quality check of the final library before sequencing helps sample normalization and to avoid potential sequencing over-amplification artifacts that may occur during PCR amplification.

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GCF provides quality control service using the Agilent Tapestation system for both DNA and RNA and quantification using the Qubit assays or NanoDrop.

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Here you find some useful tips about RNA/DNA preparation and quality control for genomic studies.