CRYPTO-POC
Low-cost point-of care cryptosporidiosis test in children in Ethiopia (CRYPTO-POC)
Main content
The primary objective of this study was to estimate the accuracy and operational performance of simple microscopy of auramine stained stool samples for diagnosis of Cryptosporidium infection. Secondary aims were to evaluate risk factors for Cryptosporidium infection and shedding over time. Approximately 1000 Ethiopian children below 5 years presenting to Jimma University Hospital or and Serbo health center with diarrhea over a 12-month enrollment period were included. Additionally, 750 healthy controls from health centers in the same area were also included. Clinical data, HIV test and a stool sample were collected and analyzed. The study was conducted between December 2016 and July 2018. Some analyses of microbiome data are still being analyzed.
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Published results from the study showed that:
LED-AP has high sensitivity and specificity for cryptosporidiosis and should be considered as a dual-use technology that can be easily integrated with existing laboratory infrastructures in low-resource settings.
On average, children infected with Cryptosporidium continue to shed parasite oocysts in their stools for 31 days after the diarrhea episode started.
Diarrhea cases are associated with lower bacterial diversity and enriched in Escherichia spp., Campylobacter spp., and Streptococcus spp. Further, diarrhea cases are depleted in gut commensals such as P. copri, F. prausnitzii, and D. succinatiphilus, with depletion being most pronounced in prolonged/persistent cases.
Publications from the CryptoPOC study
Zangenberg M, Johansen 脴H, Abdissa A, Eshetu B, Kurtzhals JAL, Friis H, Sommerfelt H, Langeland N, Hanevik K. . Trop Med Int Health. 2019 Sep;24(9):1088-1097. doi: 10.1111/tmi.13291. Epub 2019 Aug 16. PMID: 31325406.
Johansen 脴H, Abdissa A, Zangenberg M, Mekonnen Z, Eshetu B, Bj酶rang O, Alemu Y, Sharew B, Langeland N, Robertson LJ, Hanevik K. Lancet Infect Dis. 2021 May;21(5):722-730. doi: 10.1016/S1473-3099(20)30556-9.
Johansen 脴H, Abdissa A, Zangenberg M, Mekonnen Z, Eshetu B, Sharew B, Moyo S, Sommerfelt H, Langeland N, Robertson LJ, Hanevik K. . PLoS Negl Trop Dis. 2022 Jun 6;16(6):e0010508. doi: 10.1371/journal.pntd.0010508.
Johansen 脴H, Abdissa A, Bj酶rang O, Zangenberg M, Sharew B, Alemu Y, Moyo S, Mekonnen Z, Langeland N, Robertson LJ, Hanevik K. . Microbiol Spectr. 2022 Aug 31;10(4):e0274121. doi: 10.1128/spectrum.02741-21.
Alemu Y, Abdissa A, Mekonnen Z, Sharew B, Johansen 脴H, Bj酶rang O, Langeland N, Hanevik K, Moyo SJ; CRYPTO-POC study group. . Parasitol Res. 2023 Dec 13;123(1):38. doi: 10.1007/s00436-023-08029-5
Tesfaw G, Siraj DS, Abdissa A, Jakobsen RR, Johansen 脴H, Zangenberg M, Hanevik K, Mekonnen Z, Langeland N, Bj酶rang O, Safdar N, Mapes AC, Kates A, Krych L, Castro-Mej铆a JL, Nielsen DS. . Nat Commun. 2024 Sep 2;15(1):7532. doi: 10.1038/s41467-024-51464-w.
