大象传媒

Luminex -Multiplexing immunoassay听

A Luminex assay is a听bead-based听immunoassay that allows for multiplex detection of up to 100 analytes simultaneously.听Color-coded microspheres, or beads, are internally dyed with different proportions of red and infrared fluorophores that correspond to a distinct spectral signature, or bead region. The quantification of multiple cytokines and other biomarkers in a sample provides critical information about biological processes and diseases.

Antibodies specific to a desired analyte are coupled to a unique bead region and are incubated with sample. After washing away unbound materials, samples are incubated with a mixture of biotinylated detection antibodies and a streptavidin-phycoerythrin (PE) reporter. Using a Luminex instrument, beads are excited by one laser to determine the bead region and corresponding assigned analyte. Another laser determines the magnitude of the PE-derived signal, which is proportional to the amount of analyte bound. Multiple readings are taken at each bead region, ensuring robust detection.

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Cost-effective assay for profiling multiple biomarkers markers听(over听50)听simultaneously.听

An assays require a small sample volume (<50 碌L)听conserving sample by measuring multiple analytes in a single well within a short time.听

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Pris pr plate听500NOK unsuperised.听

Supervised pris听pr plate1000NOK (Setup of machine, QC, and running the plate)听

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